Cellular heredity in haploid cultures of somatic cells, March 1968-April 1981. Final report
Technical Report
·
OSTI ID:5028219
An account is given of the development and application to cell-culture genetics of unique haploid cell lines from frog embryo developed in this laboratory. Since 1968, the main aim of this project has been to develop the haploid cell system for studies of mutagenesis in culture, particularly by ultraviolet radiation. In the course of this work we isolated chromosomally stable cell lines, derived and characterized a number of variants, and adapted cell hybridization and other methods to this material. Particular emphasis was placed on ultraviolet photobiology, including studies of cell survival, mutagenesis, and pathways of repair of uv-damaged DNA. Although at present less widely used for genetic experiments than mammalian cell lines, the frog cells offer the advantages of authentic haploidy and a favorable repertory of DNA repair pathways for study of uv mutagenesis.
- Research Organization:
- Institute for Cancer Research, Philadelphia, PA (USA). Fox Chase Cancer Center
- DOE Contract Number:
- AC02-76EV03110
- OSTI ID:
- 5028219
- Report Number(s):
- DOE/EV/03110-32; ON: DE82018433
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550300 -- Cytology
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CULTIVATION TECHNIQUES
DNA
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FROGS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAPLOIDY
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VERTEBRATES
550300 -- Cytology
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CULTIVATION TECHNIQUES
DNA
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FROGS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAPLOIDY
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VERTEBRATES