Cellular heredity in haploid cultures of somatic cells. Progress report, August 1978-September 1979. [Correlation between uv mutagenesis and DNA repair in cultured haploid frog cells (ICR 2A)]
During the past year we have continued our studies of the relation of ultraviolet mutagenesis to DNA repair in cultures of the haploid frog cell line ICR 2A. Our method of irradiation of cells in suspension was improved by construction of an improved detector with major sensitivity to the 254 nm Hg resonance line, to give better estimates of actual exposure of the cells. Using this method, dose-response and dose-fractionation studies on irradiation of ouabain resistance were carried out. The uv induction of this phenotype in the ICR 2A cell line was found to be less than that necessary for adequate analysis of dose-response curves. Cell fusion experiments using frog and mouse cells revealed an enhancement of mutagenesis in the mouse parent that will be explored in further work.
- Research Organization:
- Institute for Cancer Research, Philadelphia, PA (USA). Fox Chase Cancer Center
- OSTI ID:
- 5633971
- Report Number(s):
- COO-3110-31
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CORRELATIONS
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
FROGS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IN VITRO
INFORMATION
IRRADIATION
ISOLATED VALUES
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CORRELATIONS
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FRACTIONATED IRRADIATION
FROGS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IN VITRO
INFORMATION
IRRADIATION
ISOLATED VALUES
MUTAGENESIS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
VERTEBRATES