Cellular heredity in haploid cultures of somatic cells. Progress report, August 1977--August 1978. [Role of DNA repair mechanisms in uv mutagenesis in cultured frog and fish cells]

Technical Report · Fri Sep 01 04:00:00 EDT 1978

DOI:https://doi.org/10.2172/6776686· OSTI ID:6776686

Freed, J. J.

Studies in progress on cultured frog and fish cells, exploring the relation between the frequency of mutation after ultraviolet irradiation and the pathway through which DNA repair takes place are reported. The rationale is that the mutation frequency induced by a uv exposure is determined not only by the dose delivered but by the fidelity of the DNA repair process. Since frog cells express photoreversal enzyme, whether repair takes place by error-free photoreversal or by other, error-prone, mechanisms can be determined experimentally. An important question is whether an inducible, error-prone mutagenic form of repair is demonstrable. During the past year, methods necessary to determine uv survival and mutation frequency over a range of uv exposures were worked out. Using these methods, we have tested for alteration of the uv survival curve by previous conditioning exposures in frog cells was studied and uv survival and photoreversal capacity in fish cells were determined. The relation between uv survival and induction of ouabain resistance by an alkylating agent (MNNG) was examined as a background for further studies with uv. A procedure intended to accomplish DNA-mediated transfer of frog DNA photolyase enzyme to Chinese hamster cells is described.

• Research Organization: Institute for Cancer Research, Philadelphia, PA (USA)
• OSTI ID: 6776686
• Report Number(s): COO-3110-30
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CELL CULTURES
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ERRORS
FISHES
FROGS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENETICS
IRRADIATION
MUTAGENESIS
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
SURVIVAL TIME
ULTRAVIOLET RADIATION
VERTEBRATES