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Delayed erythropoiesis in irradiated rats grafted with syngeneic marrow: effects of cytotoxic drugs and iron-deficiency anemia. [Gamma radiation; cyclophosphamide; busulfan; dimethylmyleran]

Journal Article · · Blood; (United States)
OSTI ID:7351012
Erythropoiesis in spleens of lethally irradiated Lewis rats grafted with 4-35 x 10/sup 6/ syngeneic marrow cells was inhibited or delayed during the test period of 5 days; this was in marked contrast to observations in irradiated mice. The mechanism of this inhibition was the subject of this study. Pretreatment of recipients 9 days prior to irradiation with the cytotoxic drugs cyclophosphamide (CY), busulfan (BUS), or dimethylmyleran (DMM), or the induction of iron deficiency with anemia in recipients reversed this delayed erythropoiesis. However, neither iron-deficiency anemia nor pretreatment with BUS or DMM affected the ability of irradiated recipients to reject 20 to 50 x 10/sup 6/ allogeneic marrow cells. The administration of commercial preparations of erythropoietin to hosts stimulated erythropoiesis moderately. However, proliferation of syngeneic marrow cells was not enhanced when infused into lethally irradiated spontaneous hypertensive (SH) inbred-strain rats which have high levels of endogenous erythropoietin. Finally, plasma from irradiated rats treated with phenylhydrazine to produce severe anemia was rich in erythropoietin but failed to stimulate erythropoiesis in the cell transfer system. Two hypotheses are considered.
Research Organization:
Boston Univ.
OSTI ID:
7351012
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 48:3; ISSN BLOOA
Country of Publication:
United States
Language:
English