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Marrow transplantation in the treatment of a murine heritable hemolytic anemia

Journal Article · · Blood; (United States)
OSTI ID:5901496
;
Mice with hemolytic anemia, sphha/sphha, have extremely fragile RBCs with a lifespan of approximately one day. Neither splenectomy nor simple transplantation of normal marrow after lethal irradiation cures the anemia but instead causes rapid deterioration and death of the mutant unless additional prophylactic procedures are used. In this report, we show that normal marrow transplantation preceded by sublethal irradiation increases but does not normalize RBC count. The mutant RBCs but not all the WBCs are replaced by donor cells. Splenectomy of the improved recipient causes a dramatic decrease in RBC count, indicating that the mutant spleen is a site of donor-origin erythropoiesis as well as of RBC destruction. Injections of iron dextran did not improve RBC counts. Transplantation of primary recipient marrow cells into a secondary host with a heritable stem cell deficiency (W/Wv) corrects the defect caused by residence of the normal cells in the sphha/sphha host. The original +/+ donor cells replace the RBCs of the secondary host, and the RBC count is normalized. Results indicate that the environment in the sphha/sphha host is detrimental to normal (as well as mutant) erythroid cells but the restriction is not transmitted.
Research Organization:
Jackson Laboratory, Bar Harbor, ME (USA)
OSTI ID:
5901496
Journal Information:
Blood; (United States), Journal Name: Blood; (United States) Vol. 73:7; ISSN BLOOA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COUNT
BLOOD FORMATION
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
DISEASES
ENZYMES
ERYTHROCYTES
ERYTHROPOIESIS
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
IRRADIATION
ISOMERASES
LETHAL IRRADIATION
LIFE SPAN
MAMMALS
MATERIALS
MEDICINE
MICE
MOSAICISM
ORGANS
PHENOTYPE
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SPLEEN
SPLENECTOMY
STEM CELLS
SURGERY
SYMPTOMS
THERAPY
TISSUES
TRANSPLANTS
VERTEBRATES