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Evaluation of stem cell reserve using serial bone marrow transplantation and competitive repopulation in a murine model of chronic hemolytic anemia

Journal Article · · Exp. Hematol.; (United States)
OSTI ID:7071259
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Serial transplantation and competitive repopulation were used to evaluate any loss of self-replicative capacity of bone marrow stem cells in a mouse model with increased and persistent hemopoietic demands. Congenic marrows from old control and from young and old mice with hereditary spherocytic anemia (sphha/sphha) were serially transplanted at 35-day intervals into normal irradiated recipients. Old anemic marrow failed or reverted to recipient karyotype at a mean of 3.5 transplants, and young anemic marrow reverted at a mean of 4.0 transplants, whereas controls did so at a mean of 5.0 transplants. In a competitive assay in which a mixture of anemic and control marrow was transplanted, the anemic marrow persisted to 10 months following transplantation; anemic marrow repopulation was greater if anemic marrow sex matched with the host. It is possible that lifelong stress of severe anemia decreases stem cell reserve in the anemic sphha/sphha mouse marrow. However, marginal differences in serial transplantation number and the maintenance of anemic marrow in a competition assay would suggest that marrow stem cells, under prolonged stress, are capable of exhibiting good repopulating and self-replicating abilities.
Research Organization:
Univ. of Washington School of Medicine, Seattle (USA)
OSTI ID:
7071259
Journal Information:
Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 16:8; ISSN EXHMA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MODELS
BODY
BONE MARROW
CELL CYCLE
CELL PROLIFERATION
CHIMERAS
DISEASES
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
KARYOTYPE
MAMMALS
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
SYMPTOMS
TISSUES
TRANSPLANTS
VERTEBRATES