Radiation response of cell populations irradiated in situ and separated from a fibrosacroma

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574467· OSTI ID:7343030

Grdina, D J; Basic, I; Guzzino, S; Mason, K A

A cell separation system involving the centrifugation of cells in linear density gradients of Renografin provides a useful method for the in vivo study of the various clonogenic subpopulations comprising a solid tumor. Following this procedure, two selected elonogenic cell populations were separated from a murine fibrosarcoma after exposure in situ to ionizing radiation. The clonogenicity of each population (Band 2,anti rho = 1.08 g/cm/sup 3/ and Band 4,anti rho = 1.14 g/cm/sup 3/) was determined using a lung colony assay. Survival curves were constructed and compared with data from an unseparated population. The Band 2 population, when irradiated in air-breathing animals, had a relatively large fraction of sensitive cells and a more resistant component whose response was apparent at doses greater than 1000 rad. The D/sub 0/ of this resistant fraction was 459 rad. Comparing the survival curves of Band 4 and unseparated cells, the values of D/sub 0/ were similar (403 and 413 rad, respectively), but the parameters describing the shoulder region of these curves differed significantly (Band 4, D/sub q/ = 644 rad, n = 5.2; and unseparated cells, D/sub q/ = 344 rad, n = 2.3). Under conditions of acute hypoxia, Band 2 cells became significantly more radioresistant. No change in radiation sensitivity was apparent, however, for Band 4 cells. It was concluded that the Band 4 population probably consisted primarily of chronically hypoxic cells since conditions of acute hypoxia had little, in any, effect on their response to radiation.

• Research Organization: Univ. of Texas Systems Cancer Center, Houston
• OSTI ID: 7343030
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 66:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CESIUM 137
CESIUM ISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FIBROSARCOMAS
GAMMA RADIATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
MAMMALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RODENTS
SARCOMAS
TUMOR CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES