Radiation response of clonogenic cell populations separated from a fibrosarcoma

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · Radiat. Res., v. 63, no. 3, pp. 483-493

DOI:https://doi.org/10.2307/3574100· OSTI ID:4157038

Grdina, D J; Basic, I; Mason, K A; Withers, H R

Five populations of cells from a murine fibrosarcoma were separated in continuous gradients of Renografin in the density range of 1.05 to 1.18 g/cm$sup 3$. The clonogenicity of these cells was determined using a lung colony assay. The cell populations collected at densities between 1.05 and 1.09 were the more clonogenic. The radiation response of unseparated control cells and cells in each of the five separated populations was studied. The D$sub 0$'s ranged from 132 to 207 rad, the larger values being associated with the denser populations. The extrapolation numbers also varied; the higher values were found for the lighter populations. Only the population of cells banding at a density of 1.08 showed a response similar to that of the control, and this population was found to be the most clonogenic. (auth)

• Research Organization: Univ. of Texas, Houston
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-007529
• OSTI ID: 4157038
• Journal Information: Radiat. Res., v. 63, no. 3, pp. 483-493, Journal Name: Radiat. Res., v. 63, no. 3, pp. 483-493; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

*CLONE CELLS-- SURVIVAL CURVES
*FIBROSARCOMAS-- BIOLOGICAL RADIATION EFFECTS
*TUMOR CELLS-- RADIOSENSITIVITY
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
CESIUM 137
GAMMA RADIATION
IN VITRO
IRRADIATION
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SURVIVAL TIME
WHOLE-BODY IRRADIATION