Radiation sensitivity of murine fibrosarcoma cells separated by centrifugal elutriation

Journal Article · Thu Nov 01 04:00:00 EST 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575068· OSTI ID:5558155

Hunter, N ^[1]; Peters, L J; Grdina, D J; White, R A; Bartel, A

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1. Univ. of Texas System Cancer Center, Houston

Murine fibrosarcoma cells from primary asynchronous cultures were separated and partially synchronized on the basis of cell size by centrifugal elutriation. The cell size distribution of each elutriator fraction was determined with a Coulter counter, and cell-cycle parameters were derived from DNA histograms obtained by flow microfluorometry and from autoradiogarphy. The proportion of clonogenic cells from each elutriator fraction surviving 600 rad of ..gamma.. irradiaton under aerobic conditions was determined using a lung colony assay in whole-body irradiated mice. The efficiency of the assay was standardized by adding 10/sup 6/ lethally irradiated, unseparated tumor cells to all inocula. The 600-rad surviving fraction was also determined for the original unseparated population (prior to elutriation) and for a reconstituted population composed of proportional aliqots of the elutriated fractions. These latter assays gave similar results, while the surviving fraction of elutriated subpopulations differed by a factor of 3. The age-response curve generated by this method for these fibrosarcoma cells suggests that there is a compartment of radioresistant G/sub 1/ cells as well as a relatively sensitive S-cell compartment.

• OSTI ID: 5558155
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 80:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
CENTRIFUGATION
CLONING
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ELUTRIATION
FIBROSARCOMAS
GAMMA RADIATION
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MICE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RODENTS
SARCOMAS
SEPARATION PROCESSES
TUMOR CELLS
VERTEBRATES