Heterogeneity in cytokinetic, clonogenic, and radiation response induced by nutrient deprivation

Technical Report · Wed Jan 01 04:00:00 EST 1986

DOI:https://doi.org/10.2172/5925488· OSTI ID:5925488

Grdina, D J ^[1]; Williamson, K D; Johnson, T S ^[2]; Sigdestad, C P ^[3]

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1. Argonne National Lab., IL (USA)
2. Anderson (M.D.) Hospital and Tumor Inst., Houston, TX (USA)
3. Louisville Univ., KY (USA). Dept. of Radiation Oncology

A murine fibrosarcoma cell line (FSA 1233), with a capability to grow in vitro, was used to study biophysical heterogeneity induced by nutrient deprivation. Cell sub-populations were isolated by centrifuging cells through linear Renografin-density gradients. The effects of cell growth to late plateau phase on the cytokinetic, clonogenic, and radiation-survival characteristics were studied. It was observed that with increasing age of the cell culture, an increase in the proportion of relatively dense cells was associated with a decrease in plating efficiency. Cells collected at p=1.08 g/cm{sup 3} have a plating efficiency of 0.2% compared with 0.01% for those isolated at p=1.16 g/cm{sup 3}. Discrepancies were also seen between the tritiated thymidine labeling index (LI) and flow cytometry (FCM, % S phase) for cells collected throughout the gradient. The radiation sensitivities of selected cell populations isolated by density gradient centrifugation were determined and compared with data from a control plateau phase population. 15 refs., 4 figs., 1 tab.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: DOE; DOHHS; USDOE, Washington, DC (USA); Department of Health and Human Services, Washington, DC (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5925488
• Report Number(s): ANL/PP-66846; ON: DE91011122; CNN: CA37435
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CELL CYCLE
CELL FLOW SYSTEMS
CENTRIFUGATION
DISEASES
EXPERIMENTAL NEOPLASMS
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
MAMMALS
MICE
NEOPLASMS
NUTRIENTS
NUTRITIONAL DEFICIENCY
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RODENTS
SARCOMAS
SEPARATION PROCESSES
SURVIVAL CURVES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
ULTRACENTRIFUGATION
VERTEBRATES