Inhibition of host cell protein synthesis by UV-inactivated poliovirus
The ability of poliovirus that was irradiated with UV light at energies up to 2,160 ergs/mm/sup 2/ to subsequently inhibit host cell protein synthesis was measured. The inactivation of the host cell shutoff function followed one-hit kinetics. Increasing irradiation did not affect the rate of inhibition until the multiplicity of infection after irradiation was reduced to approximately 1 PFU/cell. At higher functional multiplicities, the rate was unchanged, but an increasing lag before the onset of inhibition was observed with increasing irradiation. The energy levels required to inactivate virus-induced inhibition of host cell protein synthesis suggest that damage to virus RNA rather than to virus capsid proteins is responsible for the loss of function. When the inactivation of host cell shutoff was compared with the inactivation of other viral functions by UV irradiation, it correlated exactly with the loss of infectivity but not with other viral functions measured. Guanidine treatment, which prevents detectable viral RNA and protein synthesis, completely inhibited host cell shutoff by low multiplicities of unirradiated virus infection but not higher multiplicities. When a high multiplicity of virus was first reduced to a low titer by irradiation, host cell shutoff was still evident in the presence of guanidine. The results demonstrate that the complete inhibition of host cell protein synthesis can be accomplished by one infectious viral genome per cell.
- Research Organization:
- Univ. of Utah, Salt Lake City
- OSTI ID:
- 7330191
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 21:1; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
ELECTROMAGNETIC RADIATION
INFECTIVITY
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
POLIO VIRUS
PROTEINS
RADIATION EFFECTS
RADIATIONS
SYNTHESIS
ULTRAVIOLET RADIATION
VIRUSES