Host range restriction of vaccinia virus in Chinese hamster ovary cells: relationship to shutoff of protein synthesis. [Uv radiation]
Journal Article
·
· J. Virol.; (United States)
OSTI ID:6452577
Chinese hamster ovary cells were found to be nonpermissive for vaccinia virus. Although early virus-induced events occurred in these cells (RNA and polypeptide synthesis), subsequent events appeared to be prevented by a very rapid and nonselective shutoff of protein synthesis. Within less than 2 h after infection, both host and viral protein syntheses were arrested. At low multiplicities of infection, inhibition of RNA synthesis with cordycepin resulted in failure of the virus to block protein synthesis. Moreover, infection of the cells in the presence of cycloheximide prevented the immediate onset of shutoff after reversal of cycloheximide. Inactivation of virus particles by uv irradiation also impaired the capacity of the virus to inhibit protein synthesis. These results suggested that an early vaccinia virus-coded product was implicated in the shutoff of protein synthesis. Either the nonpermissive Chinese hamster ovary cells were more sensitive to this inhibition than permissive cells, or a regulatory control of the vaccinia shutoff function was defective.
- Research Organization:
- Universite Louis Pasteur, Strasbourg, France
- OSTI ID:
- 6452577
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 28:3; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CHEMISTRY
CYCLOHEXIMIDE
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
HAMSTERS
INHIBITION
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PESTICIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
SYNTHESIS
ULTRAVIOLET RADIATION
VACCINIA VIRUS
VERTEBRATES
VIRUSES
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
CHEMISTRY
CYCLOHEXIMIDE
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
HAMSTERS
INHIBITION
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PESTICIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RNA
RODENTS
SYNTHESIS
ULTRAVIOLET RADIATION
VACCINIA VIRUS
VERTEBRATES
VIRUSES