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Mechanism of poliovirus inactivation by ammonia

Journal Article · · J. Virol.; (United States)
OSTI ID:5140781
Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repress host translation or stimulate the synthesis of detectable amounts of viral RNA. Although ammonia has no detectable effect on naked poliovirus RNA, it causes cleavage of this RNA when still within viral particles. Therefore, the RNA genome appears to be the only component of poliovirus significantly affected by ammonia.
Research Organization:
Sandia Labs., Albuquerque, NM
OSTI ID:
5140781
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 26:2; ISSN JOVIA
Country of Publication:
United States
Language:
English