Mechanisms of poliovirus inactivation by the direct and indirect effects of ionizing radiation

Journal Article · Fri Aug 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575284· OSTI ID:6817801

Ward, R L

This study was designed to measure the effects of ionizing radiation on poliovirus particles when given under conditions where either direct (in broth) or indirect (in water) effects were predominant. Under direct conditions, inactivation of poliovirus was found to be due primarily to RNA damage, although capsid damage could account for about one-third of the viral inactivation. RNA damage did not appear to be due to strand breakage and therefore was probably caused primarily by base damage or crosslink formation. Capsid damage under direct irradiation conditions did not result in significant alterations of either the sedimentation coefficients or the isoelectric points of the poliovirus particles or detectable modification of the sizes of the viral proteins. It did, however, cause loss of availability to bind to host cells. Under indirect conditions no more than 25% of viral inactivation appeared to be due to RNA damage. However, the sedimentation coefficients and isoelectric points of the viral particles were greatly altered, and their abilities to bind to cells were lost at about three-fourths the rate of loss of infectivity. Capsid damage in this case did result in changes in the sizes of capsid proteins. Therefore, the majority of the radiation inactivation under indirect conditions appeared to be due to protein damage.

• Research Organization: Sandia Lbs., Albuquerque, NM
• OSTI ID: 6817801
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 83:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550701 -- Microbiology-- Tracer Techniques
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
COBALT 60
COBALT ISOTOPES
CROSS-LINKING
DATA
DOSES
ELECTROPHORESIS
EXPERIMENTAL DATA
HELA CELLS
INACTIVATION
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
POLIO VIRUS
POLYMERIZATION
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RIBOSIDES
RNA
STRAND BREAKS
SURVIVAL CURVES
TRITIUM COMPOUNDS
VIRUSES
YEARS LIVING RADIOISOTOPES