Inactivation of poliovirus in digested sludge
- Sandia Labs., Albuquerque, NM
The effect of anaerobically digested sludge on poliovirus during incubation at temperatures between 28 and 4/sup 0/C was studied. Although virus was fully recoverable from sludge, its infectivity decreased in proportion to the time and temperature of incubation. The rate ranged from greater than 1 log per day at 28/sup 0/C to about 1 log every 5 days at 4/sup 0/C. The mechanism of inactivation was studied at the lower temperature where the sedimentation coefficients of most inactivated particles were not detectably modified. The ribonucleic acid (RNA) of these particles appeared to have been nicked and had an average sedimentation value about 70 percent that of RNA from infectious virus. Since the specific infectivity of RNA from particles recovered from sludge was directly proportional to that of the particles from which it was extracted, loss of infectivity was probably due to inactivation of RNA. Some breakdown was also found in the two largest viral proteins of inactivated particles. Thus, the mechanism of inactivation may be cleavage of viral proteins followed by nicking of encapsulated RNA. Because no virucidal activity was found in raw sludge, this component of digested sludge appears to be a product of the digestion process.
- OSTI ID:
- 7342734
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 31:6; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
552000 -- Public Health
59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
ANAEROBIC DIGESTION
BIOCONVERSION
DIGESTION
HYDROGEN COMPOUNDS
INACTIVATION
INFECTIVITY
LIQUID WASTES
MANAGEMENT
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PARTICLES
POLIO VIRUS
PROCESSING
PROTEINS
RNA
SEWAGE
SEWAGE SLUDGE
TEMPERATURE EFFECTS
VIRUSES
WASTE MANAGEMENT
WASTE PROCESSING
WASTE WATER
WASTES
WATER