Radioimmunoassay of IgG and IgM rheumatoid factors reacting with human IgG. [/sup 125/I tracer technique]

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · J. Immunol.; (United States)

OSTI ID:7323971

Carson, D A; Lawrance, S; Catalano, M A; Vaughan, J H; Abraham, G

Although IgG rheumatoid factor may play a central role in the pathogenesis of rheumatoid arthritis, previously there have been no precise methods for its specific measurement in serum and synovial fluid. This paper describes a solid phase radioimmunoassay for the independent quantification of IgM and IgG rheumatoid factor reacting with the Fc fragment of human IgG. As measured by this assay, serum IgG rheumatoid factor levels differed significantly between patients with seropositive and seronegative rheumatoid arthritis and normal control subjects. In addition, several sera and joint fluids from patients with seropositive rheumatoid arthritis, even without vasculitis, were shown by gel chromatography to have acid-dissociable complexes of IgG rheumatoid factor suggestive of IgG-IgG dimer or trimer formation.

• Research Organization: Scripps Clinic and Research Foundation, La Jolla, CA
• OSTI ID: 7323971
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 119:1; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RHEUMATIC DISEASES
SEPARATION PROCESSES
SKELETAL DISEASES
TRACER TECHNIQUES