Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis. Radioimmunoassay with monoclonal rheumatoid factor. [/sup 125/I tracer technique]

Conference · Fri Aug 01 00:00:00 EDT 1975 · J. Clin. Invest.; (United States)

OSTI ID:7352734

Luthra, H S; McDuffie, F C; Hunder, G G; Samayoa, E A

Evidence for the presence of immune complexes in blood, synovial fluid, and tissues of patients with rheumatoid arthritis (RA) includes low complement levels in blood and effusions, deposition of immunoreactants in tissues and vessel walls, and precipitate formation after addition of monoclonal rheumatoid factor (mRF) to serum or synovial fluid. To quantitate immune complex-like material in RA patients, we developed a radioimmunoassay based on inhibition by test samples of the interaction of (/sup 125/I) aggregated IgG (aggIgG) and mRF coupled to cellulose. This method could measure immune complexes of human antibody with hemocyanin prepared in vitro. The assay was not influenced by presence of polyclonal RF in test samples, nor by freezing and thawing.

• Research Organization: Mayo Foundation, Rochester, MN
• OSTI ID: 7352734
• Journal Information: J. Clin. Invest.; (United States), Vol. 56:2; Conference: 6. Pan-American congress on rheumatic diseases, Toronto, Ont., Canada, 18 Jun 1974
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
RADIOIMMUNOASSAY
BONE JOINTS
RHEUMATIC DISEASES
COMPLEXES
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BLOOD
BLOOD SERUM
BODY FLUIDS
HEMOCYANIN
IODINE 125
PATIENTS
TRACER TECHNIQUES
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BIOLOGICAL MATERIALS
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INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOISOTOPES
SKELETAL DISEASES
SKELETON
550601* - Medicine- Unsealed Radionuclides in Diagnostics