Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis. Radioimmunoassay with monoclonal rheumatoid factor. [/sup 125/I tracer technique]
Conference
·
· J. Clin. Invest.; (United States)
OSTI ID:7352734
Evidence for the presence of immune complexes in blood, synovial fluid, and tissues of patients with rheumatoid arthritis (RA) includes low complement levels in blood and effusions, deposition of immunoreactants in tissues and vessel walls, and precipitate formation after addition of monoclonal rheumatoid factor (mRF) to serum or synovial fluid. To quantitate immune complex-like material in RA patients, we developed a radioimmunoassay based on inhibition by test samples of the interaction of (/sup 125/I) aggregated IgG (aggIgG) and mRF coupled to cellulose. This method could measure immune complexes of human antibody with hemocyanin prepared in vitro. The assay was not influenced by presence of polyclonal RF in test samples, nor by freezing and thawing.
- Research Organization:
- Mayo Foundation, Rochester, MN
- OSTI ID:
- 7352734
- Conference Information:
- Journal Name: J. Clin. Invest.; (United States) Journal Volume: 56:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD SERUM
BODY
BODY FLUIDS
BONE JOINTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEMOCYANIN
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RHEUMATIC DISEASES
SKELETAL DISEASES
SKELETON
TRACER TECHNIQUES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD SERUM
BODY
BODY FLUIDS
BONE JOINTS
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEMOCYANIN
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RHEUMATIC DISEASES
SKELETAL DISEASES
SKELETON
TRACER TECHNIQUES