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Demonstration onf anticollagen antibodies in rheumatoid arthritis synovial fluids by 14C-radioimmunoassay

Journal Article · · Arthritis Rheum.; (United States)
; ; ; ;

Twenty-seven synovial fluids from rheumatoid arthritis (RA) patients and 17 synovial fluids from controls were investigated in a new radioimmunoassay for anticollagen antibodies. In vitro labeled human 14C-collagen of type I in native or denatured state was used as antigen. Passive hemagglutination was used in comparison. Parameters for defining positive results in radioimmunoassay were evaluated on the basis of control synovial fluids. Synovial fluids from 20 RA patients (74%) showed antibodies to denatured collagen; synovial fluids from 8 RA patients (30%) also demonstrated antibodies to native collagen. Control fluids of posttraumatic effusions were negative; among the other controls synovial fluid from 1 psoriatic arthritis patient reacted positively. Inhibition experiments showed that antibodies to denatured collagen cross-reacted with native collagen. Inhibition with human denatured type III collagen displayed strong cross-reactivity of anti-type I collagen antibodies with type III collagen.

Research Organization:
Univ. of Vienna, Austria
OSTI ID:
5519683
Journal Information:
Arthritis Rheum.; (United States), Journal Name: Arthritis Rheum.; (United States) Vol. 21:2; ISSN ARHEA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIBODY FORMATION
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
BONE JOINTS
CARBON 14
CARBON ISOTOPES
COLLAGEN
DIAGNOSIS
DISEASES
EVEN-EVEN NUCLEI
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RHEUMATIC DISEASES
SCLEROPROTEINS
SKELETAL DISEASES
SKELETON
TRACER TECHNIQUES
YEARS LIVING RADIOISOTOPES