Persistence of collagen type II-specific T-cell clones in the synovial membrane of a patient with rheumatoid arthritis

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.2.636· OSTI ID:7266920

Londei, M; Savill, C M; Verhoef, A; Brennan, F; Leech, Z A; Feldmann, M ^[1]; Duance, V ^[2]; Maini, R N ^[3]

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1. Charing Cross Sunley Research Centre, London (England)
2. Bristol Laboratory (England)
3. Kennedy Institute of Rheumatology, London (England)

Rheumatoid arthritis is an autoimmune disease characterized by T-cell infiltration of the synovium of joints. Analysis of the phenotype and antigen specificity of the infiltrating cells may thus provide insight into the pathogenesis of rheumatoid arthritis. T cells were cloned with interleukin 2, a procedure that selects for in vivo-activated cells. All clones had the CD4 CDW29 phenotype. Their antigen specificity was tested by using a panel of candidate joint autoantigens. Four of 17 reacted against autologous blood mononuclear cells. Two clones proliferated in response to collagen type II. After 21 months, another set of clones was derived from synovial tissue of the same joint. One of eight clones tested showed a strong proliferative response against collagen type II. The uncloned synovial T cells of a third operation from another joint also responded to collagen type II. The persistence of collagen type II-specific T cells in active rheumatoid joints over a period of 3 years suggests that collagen type II could be one of the autoantigens involved in perpetuating the inflammatory process in rheumatoid arthritis.

• OSTI ID: 7266920
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:2; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGENS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE JOINTS
CELL FLOW SYSTEMS
CELL PROLIFERATION
COLLAGEN
CONNECTIVE TISSUE CELLS
DISEASES
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
INFLAMMATION
IRRADIATION
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PATHOLOGICAL CHANGES
PATIENTS
PROTEINS
PYRIMIDINES
RHEUMATIC DISEASES
RIBOSIDES
SCLEROPROTEINS
SKELETAL DISEASES
SKELETON
SOMATIC CELLS
SYMPTOMS
THYMIDINE
TRITIUM COMPOUNDS