Radioimmunoassay of sisomicin

Journal Article · Sun Feb 01 04:00:00 EST 1976 · Antimicrob. Agents Chemother.; (United States)

DOI:https://doi.org/10.1128/AAC.9.2.247· OSTI ID:7322959

Broughton, A; Strong, J E; Bodey, G P

An antibody induced in rabbits against a gentamicin-bovine serum albumin conjugate cross-reacted with the chemically related aminoglycoside, sisomicin, facilitating the development of a sisomicin radioimmunoassay. Sisomicin was labeled with /sup 125/I using an acylating agent (3-(4-hydroxyphenyl)propionic acid-N-hydroxy-succinimide ester). The resulting assay produced a linear standard curve on a logit-log plot with a sensitivity of 140 pg. Comparison with a microbiological assay showed no significant difference (P less than 0.001) in the measurement of sisomicin by these two methods.

• Research Organization: Univ. of Texas, Houston
• OSTI ID: 7322959
• Journal Information: Antimicrob. Agents Chemother.; (United States), Journal Name: Antimicrob. Agents Chemother.; (United States) Vol. 9:2; ISSN AMACC
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBIOTICS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES