Radioimmunoassay of iodinated tobramycin

Journal Article · Fri Oct 01 04:00:00 EDT 1976 · Antimicrob. Agents Chemother. (1961-70); (United States)

DOI:https://doi.org/10.1128/AAC.10.4.652· OSTI ID:7215391

Broughton, A; Strong, J E; Pickering, L K; Bodey, G P

A radioimmunoassay for tobramycin was developed using antisera from rabbits injected with a tobramycin-bovine serum albumin conjugate. The antibody had a relatively high affinity for /sup 125/I-labeled tobramycin (1.6 x 10/sup 9/ liters/mol), thus indicating little interference in immunoreactivity by iodination. The radioimmunoassay was highly specific, and only minor cross-reaction occurred with other aminoglycoside antibiotics. The radioimmunoassay was also more sensitive than previously published tobramycin assay (minimum sensitivity, 280 pg), and there was a significant correlation (P < 0.05) with both the radioenzymatic and microbiological assays.

• Research Organization: Univ. of Texas, Houston
• OSTI ID: 7215391
• Journal Information: Antimicrob. Agents Chemother. (1961-70); (United States), Journal Name: Antimicrob. Agents Chemother. (1961-70); (United States) Vol. 10:4; ISSN AACHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES