Radioimmunoassay for pantothenic acid in blood and other tissues. [/sup 3/H and /sup 14/C tracer techniques]
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:6433811
We described a radioimmunoassay for pantothenic acid in biological tissues. D-Pantothenic acid was conjugated with bovine serum albumin by use of a bromoacetyl derivative of pantothenic acid, and antibody to this antigen was raised by injecting it into the foot pads of rabbits. For the radioimmunoassay, a 100-fold dilution of the resulting antiserum was incubated with radiolabeled panthothentic acid. The antibodies were precipitated and dissolved, and the radioactivity of the solution was measured in a liquid scintillation counter. Between 5 and 125 ng of pantothenic acid can be detected in 75 ..mu..L of tissue extract. Validation included recovery and precision studies, parallelism with tissue extracts, and competitive binding studies. Results of the radioimmunoassay and those of microbiological assay with use of Lactobacillus plantarum correlated well (r = 0.80).
- Research Organization:
- Utah State Univ., Logan
- OSTI ID:
- 6433811
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 25:1; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
EVEN-EVEN NUCLEI
HYDROGEN ISOTOPES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PANTOTHENIC ACID
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
EVEN-EVEN NUCLEI
HYDROGEN ISOTOPES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PANTOTHENIC ACID
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
VITAMIN B GROUP
VITAMINS
YEARS LIVING RADIOISOTOPES