Radioimmunoassay for zearalenone and zearalanol in human serum: production, properties, and use of porcine antibodies

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5504736

Thouvenot, D; Morfin, R F

To produce antigens susceptible to raise antibodies for resorcylic acid lactones, the 6'-carboxymethyloxime derivatives of zearalenone and zearalanone were bound to bovine serum albumin. Pigs could be immunized by using these antigens, the best titer in antibodies being obtained with the zearalenone antigen. The procine antibodies were specific for the resorcylic acid lactones of structural resemblance with zearalenone. This specificity made the antibodies usable for a radioimmunoassay of zearalenone and zearalanol, which may be found in human and animal sera. The range of the assay was between 0.25 and 10 ng. The limit of detection was 5 ppb (5 ng/ml) in human serum.

• Research Organization: Faculte des Sciences, Brest, France
• OSTI ID: 5504736
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 45:1; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BIOCHEMISTRY
BLOOD SERUM
CHEMISTRY
DOMESTIC ANIMALS
FUNGI
FUSARIUM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
PARASITES
PATIENTS
PLANTS
RADIOASSAY
RADIOIMMUNOASSAY
SPECIFICITY
SWINE
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES