Sensitive radioimmunoassay for detection of antibodies to recombinant human interferon-alpha A

Journal Article · Sun Nov 30 23:00:00 EST 1986 · J. Interferon Res.; (United States)

DOI:https://doi.org/10.1089/jir.1986.6.705· OSTI ID:6409021

Palleroni, A V; Trown, P W

A radioimmunoassay (RIA) for the detection of antibodies to recombinant human leukocyte interferon A (rHuIFN-alpha A) in human serum has been developed and validated against the standard antiviral neutralization bioassay (ANB). The assay measures the binding of /sup 125/I-labeled rHuIFN-alpha A to immunoglobulins in serum. Aliquots of patients' sera are incubated with /sup 125/I-rHuIFN-alpha A and the complexes formed between antibodies in the sera and the /sup 125/I-rHuIFN-alpha A are precipitated with goat anti-human IgG serum. The radioactivity in the immune precipitate is a measure of the quantity of antibody (if present) in the serum. The sensitivity of this RIA is 5 ng of IgG/ml of serum.

• Research Organization: Hoffmann-La Roche Inc., Nutley, NJ
• OSTI ID: 6409021
• Journal Information: J. Interferon Res.; (United States), Journal Name: J. Interferon Res.; (United States) Vol. 6; ISSN JIRED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GROWTH FACTORS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INTERFERON
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYMPHOKINES
MAMMALS
MAN
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
VERTEBRATES