Quantitative radiommunoassay for DNA-binding antibodies. [Iodine 131, Iodine 125]

Journal Article · Tue Sep 01 04:00:00 EDT 1981 · J. Lab. Clin. Med.; (United States)

OSTI ID:6012458

Smith, L H; Guyer, R L; Minami, R M; Teplitz, R L

A radioimmunoassay (RIA) is described for the measurement of serum immunoglobulins capable of binding to double-standard or single-standard DNA. DNA attached to Sephadex G-50 by ultraviolet radiation was used as a solid- phase immunoabsorbent for DNA-binding proteins from serum. Goat anti-human (GAH) IgG (/sup 125/I-labeled) were used to detect the human immunoglobulins bound onto the washed DNA-Sephadex. The quantities of immunoglobulins bound were determined by comparison with a standard curve constructed by dilution of a plasma from an systemic lupus erythematosus (SLE) patient containing known amounts of bound, DNA-specific IgM and IgG. Another RIA was employed for measuring levels of IgG and IgM. In combination with measurements of the total serum IgM and IgG, the RIA allowed for the determination of the fraction of the total serum IgM or IgG that was specific for double- or single-standard DNA. For a pool of normal human sera the quantities were as follows: 0.04% of the total IgM and 0.001% of the total IgG bound double-standard DNA; 0.22% of the total IgM and 0.05% of the total IgG bound single-stranded DNA. This capability is important because information regarding the quantitative measurement of antibodies to DNA and their class determination may be of significance in monitoring the status of subjects with SLE.

• Research Organization: City of Hope National Medical Center, Duarte, CA
• OSTI ID: 6012458
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 98:3; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ERYTHEMA
GLOBULINS
IMMUNE SERUMS
IMMUNOGLOBULINS
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LUPUS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SKIN DISEASES
SYMPTOMS
TRACER TECHNIQUES
TUBERCULOSIS
ULTRAVIOLET RADIATION