Differential immunoadsorption coupled with rate nephelometry for estimation of DNA-binding immunoglobulins

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6412109

DeBari, V A; Nicotra, J; Blaney, J F; Schultz, E F; Needle, M A

The authors describe a technique for estimating the mass of anti-DNA antibodies by immunonephelometry of serum immunoglobulins (IgG, IgA, IgM) before and after adsorption onto DNA bound to agarose-polylysine columns. Sixteen patients with systemic lupus erythematosus and 16 age- and sex-matched controls were studied. Precision was determined for high-value (in 10 patients) and low-value (in nine controls) ranges for each of the immunoglobulins. They found anti-DNA antibody concentrations (mean +/- SD) in systemic lupus erythematosus of 1.981 +/- 1.015 g/L for IgG 0.257 +/- 0.215 g/L for IgA and 0.282 +/- 0.234 g/L for IgM. Sensitivity and linearity are such that fivefold dilutions of patients' serum with either a buffered albumin solution or control serum yielded values close to the expected values for IgG. Similarly diluted sera gave inordinately high values in the radiometric binding assay. Neither parametric (linear regression) nor nonparametric correlation methods (Spearman's rank and Kendall's tau) show a significant correlation between patients' data obtained by the present technique and that by a radiometric binding assay, although combined data from patients and controls demonstrate a significant nonparametric correlation.

• Research Organization: Univ. of Medicine and Dentistry of New Jersey, Paterson
• OSTI ID: 6412109
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 30:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BACTERIAL DISEASES
BLOOD SERUM
CHEMICAL ANALYSIS
DISEASES
DNA
GLOBULINS
IMMUNOGLOBULINS
INFECTIOUS DISEASES
LUPUS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOMETRIC ANALYSIS
SKIN DISEASES
TUBERCULOSIS