Role of serum IgA. Hepatobiliary transport of circulating antigen
The IgA mediated hepatobiliary excretion of antigen from the circulation was studied using a radiolabeled haptenated protein (dinitrophenyl-human serum albumin) injected intravenously in mice together with monoclonal anti-dinitrophenyl antibodies of different immunoglobulin classes. Antibodies were obtained from ascitic fluids of mice bearing the MOPC315 myeloma (IgA), or immune spleen cell hybridomas (IgG and IgM). IgA antibody brought about the transport of large amounts of antigen from the circulation to the bile during 1-3h. Analysis of bile by gel filtration showed that a large part of the transported antigen remained intact and complexed with IgA. Neither IgA of different specificity nor anti-dinitrophenyl IgM medicated biliary transport of antigen. With anti-dinitrophenyl IgG, only small amounts of low molecular weight fragments of labeled antigen were found in he bile. Preformed immune complex of radiolabeled antigen and IgA antibody were rapidly transported from the circulation to the bile, resulting in threefold-higher levels of radioactivity in bile than in serum. It is proposed that an important function of serum IgA is to mediate the hepatobiliary excretion of corresponding circulating antigens.
- OSTI ID:
- 5674774
- Journal Information:
- J. Exp. Med.; (United States), Journal Name: J. Exp. Med.; (United States) Vol. 153:4; ISSN JEMEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
IgA on the surface of erythrocytes from IgA nephropathy patients
Platelet-collagen interaction: inhibition by a monoclonal antibody raised against collagen receptor
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BILIARY TRACT
BLOOD SERUM
BODY
CLEARANCE
DIGESTIVE SYSTEM
EXCRETION
GLANDS
GLOBULINS
IMMUNOGLOBULINS
IMMUNOLOGY
IODINE ISOTOPES
ISOTOPES
LIVER
MAMMALS
MASS TRANSFER
MICE
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
VERTEBRATES