Radioimmunoassay for immunolobulin G in serum and urine

Journal Article · Sat Dec 01 04:00:00 EST 1979 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5432078

Woo, J; Floyd, M; Longley, M A; Cannon, D C

We describe a radioimmunoassay for immunoglobulin G (IgG) in serum and urine. Aliquots of diluted samples and /sup 125/I-labeled IgG were incubated in antibody-coated tubes at 37 degrees C for 24 h, the supernates were decanted, and the radioactivity in tubes containing the bound fraction was counted. The dose-response curve in the range of 0.4-500 mg/L of urine or 640-40 000 mg/L of serum was linear on logit-log transformation and iterative weighted regression. Assay sensitivity was 10 ng of IgG. Validation studies included testing for precision, accuracy, antibody specificity, and parallelism of the dose-response curves for standard and unknown. In a study of 14 apparently normal individuals, serum IgG = 4.0-10.9 gL, urine IgG = 1.1-4.8 mg/24 h, and IgG clearance = 0.2 x 10/sup -4/ to 4.8 x 10/sup -4/ mL/min. In 20 patients with renal allografts, serum IgG = 15.8-66 g/L, urine IgG = 9.6-626 mg/24 h, and IgG clearance = 9 x 10/sup -4/ to 1.99 x 10/sup -1/ mL/min. IgG values correlate well with severity of renal allograft rejection.

• Research Organization: Univ. of Texas Medical School, Houston
• OSTI ID: 5432078
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 25:12; ISSN CLCHA
• Country of Publication: United States
• Language: English

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550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SERUM
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
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IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
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IODINE ISOTOPES
ISOTOPE APPLICATIONS
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KIDNEYS
LABELLED COMPOUNDS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
URINE
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