Radioimmunoassay for urinary albumin. [/sup 125/I tracer technique]
Conference
·
· Clin. Chem.; (United States)
OSTI ID:6340499
We describe a rapid, sensitive, and precise radioimmunoassay for urinary albumin (U/sub alb/). Aliquots of diluted urine were incubated at room temperature for 1 h with /sup 125/I-labelled albumin and a rabbit antiserum monospecifid for human albumin. Phase separation was effected by the double-antibody technique. The dose-response curve was linear in the range of 15.6 to 10,000 ng, equivalent to 4 to 3000 mg/liter of urine. The limit of sensitivity was 16 ng of albumin. The coefficient of assay variation was 4.8%, both at 44 mg/liter and at 1304 mg/liter. A displacement curve obtained with a serially diluted urine sample of high albumin concentration was completely superimposable with the curve for which human albumin was used as a standard. In 26 normal individuals the range for U/sub alb/ was 2.2 to 12.6 mg/24 h, and for albumin clearance (C/sub alb/), 1.8 x 10/sup -5/--19.6 x 10/sup -5/ ml/min. After renal homografts in 25 patients, U/sub alb/ ranged from 16.9 to 9928 mg/24 h, and C/sub alb/ from 2.7 x 10/sup -4/ to 1.7 x 10/sup -1/ ml/min. Both increased U/sub alb/ and C/sub alb/ correlated well with the severity of renal homograft rejection.
- Research Organization:
- Univ. of Texas Medical School, Houston
- OSTI ID:
- 6340499
- Conference Information:
- Journal Name: Clin. Chem.; (United States) Journal Volume: 24:9
- Country of Publication:
- United States
- Language:
- English
Similar Records
Radioimmunoassay for immunolobulin G in serum and urine
Beta 2-Microglobulin clearance as measured by radioimmunoassay
Evaluation of the expression of urinary selenium level as ng Se/mg creatinine and the use of single-void urine as a sample for urinary selenium determination
Journal Article
·
Fri Nov 30 23:00:00 EST 1979
· Clin. Chem. (Winston-Salem, N.C.); (United States)
·
OSTI ID:5432078
Beta 2-Microglobulin clearance as measured by radioimmunoassay
Journal Article
·
Tue Jul 01 00:00:00 EDT 1980
· Clin. Chem. (Winston-Salem, N.C.); (United States)
·
OSTI ID:5586883
Evaluation of the expression of urinary selenium level as ng Se/mg creatinine and the use of single-void urine as a sample for urinary selenium determination
Journal Article
·
Sat Aug 01 00:00:00 EDT 1981
· Bull. Environ. Contam. Toxicol.; (United States)
·
OSTI ID:6219509
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
URINE
WASTES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
URINE
WASTES