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Radioimmunoassay for metabolites of 9,3''-diacetylmidecamycin, a macrolide antibiotic

Journal Article · · Antimicrob. Agents Chemother.; (United States)
DOI:https://doi.org/10.1128/AAC.22.6.976· OSTI ID:6256889
; ; ; ;
A radioimmunoassay system has been developed for the measurement of two major metabolites of 9,3''-diacetylmidecamycin, Mb-6 and Mb-12. A radioimmunoassay for Mb-6 was performed by using anti-Mb-6 serum and a (/sup 125/I)tyramined Mb-6 derivative as a radiolabeled antigen. The labeled antigen was prepared by the chloramine T method. The antiserum was obtained from a rabbit immunized with Mb-6 conjugated to bovine serum albumin. The obtained antiserum was cross-reactive with two other metabolites of 9,3''-diacetylmidecamycin, Mb-2 and Mb-12, in addition to Mb-6. This Mb-6 radioimmunoassay system could detect Mb-6 concentrations as low as 100 pg/ml of serum. The coefficients of variation were 4.5% (intra-assay) and 5.1% (inter-assay). A radioimmunoassay for Mb-12, using anti-midecamycin serum and a (/sup 125/I)tyramined-Mb-12 derivative, has also been developed. The antiserum was cross-reactive only with Mb-12 among the 9,3''-diacetylmidecamycin metabolites. This Mb-12 radioimmunoassay system could detect Mb-12 concentrations as low as 2 ng/ml. The intra- and inter-assay variances were 5.9 and 5.8%, respectively.
Research Organization:
Tokai Laboratory, Daiichi Pure Chemicals Co. Ltd., Ibaraki, Japan
OSTI ID:
6256889
Journal Information:
Antimicrob. Agents Chemother.; (United States), Journal Name: Antimicrob. Agents Chemother.; (United States) Vol. 22:6; ISSN AMACC
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
CHEMICAL ANALYSIS
CHEMOTHERAPY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
METABOLITES
NUCLEI
ODD-EVEN NUCLEI
QUANTITATIVE CHEMICAL ANALYSIS
QUANTITY RATIO
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THERAPY
TRACER TECHNIQUES
VERTEBRATES