Radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/)

Journal Article · Mon Aug 01 04:00:00 EDT 1977 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-45-2-339· OSTI ID:7218581

Burman, K D; Strum, D; Dimond, R C; Djuh, Y Y; Wright, F D; Earll, J M; Wartofsky, L

The report describes the development of a radioimmunoassay for 3,3'-L-diiodothyronine (3,3'T/sub 2/) which may be performed on unextracted serum. Utilizing a specific antiserum to 3,3'-L-T/sub 2/-bovine serum albumin conjugates developed in rabbits, cross-reactivity was less than 0.5% with 3,5,3'-triiodothyronine (T/sub 3/) and 3,3',5'-triiodothyronine (reverse T/sub 3/) and less than 0.01% with thyroxine (T/sub 4/). Intra-assay variation averaged 2.9% and inter-assay variation was 7.8% and 18.5% when serum samples with 3,3'T/sub 2/ concentrations of 8 ng/dl and 12 ng/dl, respectively, were analyzed. Assay sensitivity was considered to be 6 ng/dl by statistical criteria. The data suggest that 3,3'T/sub 2/ circulates in the serum of normal individuals and tends to parallel serum concentrations of T/sub 3/, T/sub 4/ and rT/sub 3/ in various states of thyroid function.

• Research Organization: Walter Reed Army Medical Center, Washington, DC
• OSTI ID: 7218581
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 45:2; ISSN JCEMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
BLOOD SERUM
CARBOXYLIC ACIDS
HORMONES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PATIENTS
PEPTIDE HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
THYRONINE
TRACER TECHNIQUES