Radioimmunoassay for measurement of 3,3'-L-diiodothyronine (T/sub 2/). [/sup 125/I]

Journal Article · Wed Sep 01 04:00:00 EDT 1976 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-43-3-682· OSTI ID:7248575

Wu, S Y; Chopra, I J; Nakamura, Y; Solomon, D H; Bennett, L R

A sensitive, specific and reproducible radioimmunoassay (RIA) for measurement of 3,3'-L-diiodothyronine (T/sub 2/) in concentrated ethanol extracts of serum and amniotic fluid is described. The T/sub 2/ binding antiserum was prepared by immunization of rabbits with T/sub 2/-human serum albumin conjugate. Of various thyroid hormone derivatives tested, only 3-L-monoiodothyronine cross-reacted significantly with T/sub 2/ binding sites on the antiserum. The mean serum T/sub 2/ concentration (ng/100 ml) was 7.6 in normal subjects, 20 in hyperthyroidism, 6.0 in hypothyroidism, 7.3 in pregnancy, 7.3 in cirrhosis and 16 in newborn cord blood. T/sub 2/ concentration in amniotic fluid at term (11 ng/100 ml) was higher than that (4.1 ng/100 ml) at 15 to 16 wk of gestation. A significant increase in serum T/sub 2/ was detected following ingestion of T/sub 3/ and rT/sub 3/. These data suggest that: (a) T/sub 2/ is a normal component of human serum and amniotic fluid; (b) peripheral metabolism of T/sub 3/ as well as rT/sub 3/ contributes significantly to serum T/sub 2/ in man.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 7248575
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 43:3; ISSN JCEMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMNIOTIC FLUID
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD SERUM
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYROID HORMONES
TRACER TECHNIQUES