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3',5'-diiodothyronine in health and disease: studies by a radioimmunoassay

Journal Article · · J. Clin. Endocrinol. Metab.; (United States)
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An RIA has been developed for 3'5'-diiiodothyronine (3',5'-T/sub 2/) in unextracted serum. Interference in binding of radioactive 3',5'-T/sub 2/ to anti-3',5'-T/sub 2/ was minimized by using phosphate buffer and merthiolate. The detection threshold of the RIA was 2.5 ng/dl. Recovery of nonradioactive 3',5'-T/sub 2/ averaged 99%. T/sub 4/, T/sub 3/, and rT/sub 3/ cross-reacted with anti-3',5'-T/sub 2/ antibody 0.0025, < 0.0004, and 0.22%, respectively. The 3'-monoiodothyronine cross-reacted 1.7%. Mean serum 3',5'-T/sub 2/ concentrations in ng/dl were 2.4 in 53 normal subjects, 4.2 in 7 hypothyroid patients, 14.9 in 34 hyperthyroid patients, 13.5 in 25 patients with hepatic cirrhosis, and 14.3 in 31 newborns' cord blood serum. The values for the latter four groups were significantly different from normal. The serum 3',5'-T/sub 2/ concentration of 7.7 ng/dl in eight subjects in the third trimester of pregnancy did not differ from normal when serum T/sub 4/ and T/sub 3/ were elevated. Oral administration of 300 ..mu..g rT/sub 3/ to 9 normal subjects led to an increase in serum 3'5'-T/sub 2/ concentration of 45% at 1h. Total fasting in 3 obese subjects was associated with an increase in serum 3',5'-T/sub 2/ from 8.6 to 16.3 ng/dl at 6 to 8 days; rT/sub 3/ increased similarly, while T/sub 3/ decreased and T/sub 4/ did not change. Administration of dexamethasone to 4 hyperthyroid patients was associated with nearly parallel increases in serum 3',5'-T/sub 2/ and rT/sub 3/ and a decrease in T/sub 3/. The 3',5'-T/sub 2/ concentrations in amniotic fluids were 15.2 ng/dl at 15 to 20 weeks gestation and 5.8 ng/dl at 33 to 40 weeks. Pronase hydrolysates of 9 normal thyroid glands contained 350 ..mu..gT/sub 4/ and 0.24 ..mu..g 3',5'-T/sub 2//g wet wt. It was estimated that thyroidal secretion contributes < 1% of 3',5'-T/sub 2/ in serum of normal man.

Research Organization:
Univ. of California, Los Angeles
OSTI ID:
6009428
Journal Information:
J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 47:6; ISSN JCEMA
Country of Publication:
United States
Language:
English

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Journal Article · Wed Sep 01 00:00:00 EDT 1976 · J. Clin. Endocrinol. Metab.; (United States) · OSTI ID:7248575
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Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
AMINO ACIDS
AMNIOTIC FLUID
ANIMALS
BIOLOGICAL MATERIALS
BLOOD SERUM
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CHILDREN
COMPLEXES
CORTICOSTEROIDS
DEXAMETHASONE
DIGESTIVE SYSTEM DISEASES
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROXY ACIDS
HYDROXY COMPOUNDS
HYPERTHYROIDISM
HYPOTHYROIDISM
INFANTS
IODINE COMPLEXES
ISOTOPE APPLICATIONS
KETONES
LIVER CIRRHOSIS
MAMMALS
MAN
METABOLIC DISEASES
METABOLISM
NEONATES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PEPTIDE HORMONES
PREGNANCY
PREGNANES
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
STEROID HORMONES
STEROIDS
THYROID
THYROID HORMONES
THYRONINE
TRACER TECHNIQUES
TRIIODOTHYRONINE
VERTEBRATES