/sup 3/H and /sup 125/I radioimmunoassays of haloperidol compared with fluoroimmunoassay involving antibody coupled to magnetizable solid phase

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5639651

Rowell, F J; Hui, S M; Kamel, S R

Radioimmunoassays for haloperidol are described, involving use of tritium- or 125I-labeled drug or tritium-labeled spiroperidol, and a rabbit antiserum to a drug/bovine serum albumin conjugate. The 125I-labeled drug was prepared by the Chloramine T iodination technique. A fluoroimmunoassay for haloperidol is also described in which the antiserum is coupled to magnetizable solid-phase medium, and fluorescein-labeled haloperidol is used. The assays have acceptable accuracy, precision, and reproducibility, and are specific for haloperidol and similar butyrophenones, with no significant interference from known metabolites and other drugs. Only the radioimmunoassays have sufficient sensitivity to cover the whole range of haloperidol concentrations in serum. The fluoroimmunoassay can be used to monitor high concentrations of haloperidol in 150-microL samples or the complete concentration range of 1-mL serum samples that are extracted and concentrated before assay.

• OSTI ID: 5639651
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACCURACY
ALBUMINS
AMINES
AROMATICS
AZINES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DYES
ELECTRON CAPTURE RADIOISOTOPES
FLUORESCEIN
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
METABOLISM
METABOLITES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PIPERIDINES
POLYPHENOLS
PROTEINS
PYRIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RESOLUTION
SPECIFICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS