/sup 3/H and /sup 125/I radioimmunoassays of haloperidol compared with fluoroimmunoassay involving antibody coupled to magnetizable solid phase

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5873355

Rowell, F J; Hui, S M; Kamel, S R

Radioimmunoassays for haloperidol are described, involving use of tritium-or /sup 125/I-labeled drug or tritium-labeled spiroperidol, and a rabbit antiserum to a drug/bovine serum albumin conjugate. The /sup 125/I-labeled drug was prepared by the Chloramine T iodination technique. A fluoroimmunoassay for haloperidol is also described in which the antiserum is coupled to magnetizable solid-phase medium, and fluorescein-labeled haloperidol is used. The assays have acceptable accuracy, precision, and reproducibility, and are specific for haloperidol and similar butyrophenones, with no significant interference from known metabolites and other drugs. Only the radioimmunoassays have sufficient sensitivity to cover the whole range of haloperidol concentrations in serum. The fluoroimmunoassay can be used to monitor high concentrations of haloperidol in 150 ..mu..L samples or the complete concentration range of 1-mL serum samples that are extracted and concentrated before assay.

• Research Organization: Sunderland Polytechnic, U.K.
• OSTI ID: 5873355
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:7; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOASSAY
CENTRAL NERVOUS SYSTEM AGENTS
COMPARATIVE EVALUATIONS
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
FLUORESCENCE
HALOGEN COMPOUNDS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LUMINESCENCE
MAMMALS
MEASURING METHODS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PATIENTS
PSYCHOTROPIC DRUGS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THERAPY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES