Solid-phase radioimmunoassay for human prostatic acid phosphatase

Journal Article · Mon Sep 01 04:00:00 EDT 1975 · Cancer Res., v. 35, no. 9, pp. 2446-2451

OSTI ID:4038710

Foti, A G; Herschman, H; Cooper, J F

A solid-phase technique for radioimmunoassay of human prostatic acid phosphatase (EC 3.1.3.2) is described. Human prostatic acid phosphatase was purified from prostatic fluid. Monospecific antisera to the purified acid phosphatase were produced in rabbits. Disposable polypropylene tubes were coated with antiserum and used for radioimmunoassay with $sup 125$I-acid phosphatase. The nonspecific binding was minimized by saturating the binding sites of the tubes with bovine serum albumin. The working range of the technique was 1 to 30 ng of antigen. The solid-phase radioimmunoassay is rapid, sensitive, and efficient. In preliminary clinical trials it was shown that (a) patients with advanced prostatic cancer had elevated prostatic acid phosphatase levels by both enzymatic assay and radioimmunoassay assays, and (b) patients with other cancers were in the normal range for prostatic acid phosphatase. (auth)

• Research Organization: Southern California Permanente Medical Group, Los Angeles
• NSA Number: NSA-33-029859
• OSTI ID: 4038710
• Journal Information: Cancer Res., v. 35, no. 9, pp. 2446-2451, Journal Name: Cancer Res., v. 35, no. 9, pp. 2446-2451; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

*ACID PHOSPHATASE-- RADIOIMMUNOASSAY
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IODINE 125
LABELLED COMPOUNDS
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