Comparison of human prostatic acid phosphatase by measurement of enzymatic activity and by radioimmunoassay

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Clin. Chem.; (United States)

OSTI ID:7226295

Foti, A G ^[1]; Herschman, H; Cooper, J F

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1. Southern California Permanente Medical Group, Los Angeles

We compared results of measurement of prostatic acid phosphatase activity in serum and various tissues by enzymatic assay and radioimmunoassay. By enzymatic assay, activity in serum is lost rapidly, even at room temperature. In contrast, there was no change in antigenic activity during 48 h by radioimmunoassay. The radioimmunoassay was more specific in 12 tissues and in serum than were several enzymatic assays that make use of inhibitors of the enzyme. The enzymatic assay resulted in 26.6% (24/90) false positives from non-prostatic cancer patients. In contrast, with radioimmunoassay there were only 5.5% (5/90) false positives. We conclude that immunological detection of prostatic acid phosphatase is the more reliable technique.

• OSTI ID: 7226295
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:1; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PHOSPHATASE
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
HYDROLASES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
PHOSPHATASES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES