Serum prostate-specific acid phosphatase: development and validation of a specific radioimmunoassay. [/sup 125/I tracer technique]
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:6340643
We describe radioimmunoassay for human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified acid phosphatases from human spleen, erythrocytes, or synovial tissues. /sup 125/I-labeled acid phosphatase was prepared by a Chloramine T method, and the bound and free antigen was separated in the assay by use of anti-rabbit gamma-globulin raised in sheep. Uniform low nonspecific binding of the (/sup 125/I)acid phosphatase was achieved by using acid-phosphatase-free serum to prepare standard curves and diluted samples of serum with high acid phosphatase activities. Concentrations of immunoreactive acid phosphatase in the serum of healthy men ranged from <1 to 10 ..mu..g/liter and for 12 patients with advanced prostatic carcinoma between 100 and 500 ..mu..g/liter. The concentrations of the enzyme in sera of patients with benign prostatic hyperplasia were very similar to those in sera of the reference group.
- Research Organization:
- Univ. of Oulu, Finland
- OSTI ID:
- 6340643
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:11; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PHOSPHATASE
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
BODY
CHLORAMINES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
GLANDS
HYDROLASES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MALE GENITALS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PHOSPHATASES
PROSTATE
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID PHOSPHATASE
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD SERUM
BODY
CHLORAMINES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
GLANDS
HYDROLASES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MALE GENITALS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PHOSPHATASES
PROSTATE
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES