Radioiodinated derivatives for steroid radioimmunoassay. Application to the radioimmunoassay of cortisol

Journal Article · Fri Apr 01 04:00:00 EST 1977 · J. Lab. Clin. Med.; (United States)

OSTI ID:7312157

Gomez-Sanchez, C; Milewich, L; Holland, O B

Gamma-emitting steroid tracers for use in the radioimmunoassay of steroids have a number of advantages over the more common tritiated tracers. The steroid derivatives aldosterone-3-(p-hydroxybenzoyl) hydrazone, aldosterone-3-(p-hydroxyphenylpropionyl)hydrazone, deoxycorticosterone-3-(p-hydroxyphenylpropionyl)hydrazone, and cortisol-3-(p-hydroxyphenylpropionyl)hydrazone were synthesized by a one-step procedure and iodinated ((/sup 125/I)). To illustrate the usefulness of these derivatives, we describe the details of a cortisol radioimmunoassay. The use of the radioiodinated tracer appeared to increase the specificity of the antigen-antibody reaction when compared with (/sup 3/H)cortisol. The methodology involved in the preparation of the steroid derivatives described above can be extended to other 3-oxo-4-ene-containing steroids, with the advantages of economy, simplicity, and versatility.

• Research Organization: Univ. of Texas, Dallas
• OSTI ID: 7312157
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 89:4; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES