Radioimmunoassay of urinary free cortisol. Final report
Technical Report
·
OSTI ID:5179789
The radioimmunoassay for urinary free cortisol described in this paper is simple, rapid, and reproducible. The method uses a commercially available antibody preparation and is performed in two steps. The first step includes an extraction and a column purification to remove materials antigenically similar to cortisol from the urine. The second step is the radioimmunoassay using dextran coated charcoal to separate bound and unbound cortisol. /sup 3/H-cortisol is added prior to any mechanical manipulation to allow calculation of analytical recovery for the purification procedure. The coefficient of variation for interassay determinations was a maximum of 10.3% and for intra-assay determinations a maximum of 5.7%. Analytical recovery averaged 97.7%. One technician can analyze 100 samples per week. (Author)
- Research Organization:
- Naval Health Research Center, San Diego, CA (USA)
- OSTI ID:
- 5179789
- Report Number(s):
- AD-A-043782
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ADSORBENTS
ANTIBODIES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SUBSTITUTES
BODY FLUIDS
CARBOHYDRATES
CHARCOAL
CHROMATOGRAPHY
CORTICOSTEROIDS
DEXTRAN
EXTRACTION
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PATIENTS
POLYSACCHARIDES
PREGNANES
RADIOACTIVE MATERIALS
RADIOASSAY
RADIOIMMUNOASSAY
SACCHARIDES
SEPARATION PROCESSES
SOLVENT EXTRACTION
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
WASTES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ADSORBENTS
ANTIBODIES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD SUBSTITUTES
BODY FLUIDS
CARBOHYDRATES
CHARCOAL
CHROMATOGRAPHY
CORTICOSTEROIDS
DEXTRAN
EXTRACTION
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
PATIENTS
POLYSACCHARIDES
PREGNANES
RADIOACTIVE MATERIALS
RADIOASSAY
RADIOIMMUNOASSAY
SACCHARIDES
SEPARATION PROCESSES
SOLVENT EXTRACTION
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
WASTES