Improved radioimmunoassay of plasma cortisol

Journal Article · Thu May 01 04:00:00 EDT 1975 · Clin. Chem., v. 21, no. 6, pp. 770-773

OSTI ID:4152486

Donohue, J; Sgoutas, D

We report a simplified radioimmunoassay procedure for determination of plasma cortisol. The method offers the advantage that tracer, antibody, and assay buffer are added in a single step with the use of semi-automated equipment. Thus, critical pipetting procedures are minimized, and assay time is reduced to 4 h. No prior extraction or chromatographic purification of cortisol is required. The procedure is simple, reliable, and accurate. For either between-assay or within-assay determinations, the coefficients of variation are about 6 percent. Our results compare well with those obtained from a procedure that included extraction and chromatographic purification of cortisol in several plasma samples. Conditions for optimizing the assay are also discussed. (auth)

• Research Organization: Emory Univ., Atlanta
• NSA Number: NSA-33-003400
• OSTI ID: 4152486
• Journal Information: Clin. Chem., v. 21, no. 6, pp. 770-773, Journal Name: Clin. Chem., v. 21, no. 6, pp. 770-773; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

*HYDROCORTISONE-- RADIOIMMUNOASSAY
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
BLOOD PLASMA
IMMUNE SERUMS
MAN
N48830* --Life Sciences--New Tracer Techniques--Other
OPTIMIZATION
TRITIUM COMPOUNDS