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Simplified radioimmunoassay for plasma aldosterone

Journal Article · · Ann. Clin. Lab. Sci.; (United States)
OSTI ID:5551072
; ;

The analytical evaluation is described of a (/sup 125/I)-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negligible cross reactions with structurally-related steroids. The proposed method uses (/sup 125/I)-labeled aldosterone as the radioactive ligand. The recovery of D-aldosterone added to human plasma averaged 98 percent. The parallelism and precision of the method are excellent. Seventy specimens were assayed by the proposed method and a (/sup 3/H)-aldosterone reference procedure, which includes LH-20 column chromatographic purification prior to the radioimmunoassay step (y . 1.03X - 3.0; r . 0.99; p less than 0.01). The (/sup 3/H)-aldosterone reference procedure uses an anti-aldosterone serum prepared against aldosterone-3-oxime coupled to rabbit serum albumin.

OSTI ID:
5551072
Journal Information:
Ann. Clin. Lab. Sci.; (United States), Journal Name: Ann. Clin. Lab. Sci.; (United States) Vol. 11:2; ISSN ACLSC
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
HYDROXY COMPOUNDS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
MATERIALS
MINERALOCORTICOIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS