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A direct radioimmunoassay for aldosterone in plasma

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:6257404
; ; ;

This rapid radioimmunoassay for aldosterone is performed directly on 100 microL of unprocessed plasma, with /sup 125/I-labeled aldosterone as the labeled antigen. Researchers use of steroid-free plasma in preparing the standard curve resulted in an overestimate of aldosterone; this problem was overcome by adding to such plasma a mixture of other steroids to provide a constant steroid/aldosterone ratio. Over a wide range of aldosterone concentrations, results agreed well between the present assay and a routine method involving solvent extraction and paper chromatography (r . 0.85), and sensitivity (20 ng/L) and inter- (10.4%) and intra- (3.9%) assay CVs were better with the present assay. This assay is especially useful for multiple samples and (or) when only small-volume samples are available.

Research Organization:
Department of Endocrinology, Princess Margaret Hospital, Christchurch, New Zealand
OSTI ID:
6257404
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 29:2; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIPIDS
MATERIALS
MEASURING METHODS
MINERALOCORTICOIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
QUANTITATIVE CHEMICAL ANALYSIS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRIGLYCERIDES