Direct radioimmunoassays for ''aldosterone'' and ''18-hydroxycorticosterone'' in unprocessed urine, and their use in screening to distinguish primary aldosteronism from hypertension

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5053080

Vecsei, P ^[1]; Benraad, T J; Hofman, J; Abdelhamid, S; Haack, D; Lichtwald, K

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1. Univ. of Heidelberg, West Germany

For distinguishing primary aldosteronism from essential hypertension, we use simple direct radioimmunoassays for ''aldosterone'' (aldosterone and other materials that react with the antibody to aldosterone) and ''18-hydroxycorticosterone'' (similarly) in unprocessed urine. Patients with primary aldosteronism have high values for ''aldosterone.'' This diagnosis can be validated by assays of further urine samples from the same person and by additional direct assays for ''18-hydroxycorticosterone.'' In none of 65 urine samples from 26 patients with primary aldosteronism were both ''aldosterone'' and ''18-hydroxycorticosterone'' values within their reference intervals. However, a few ''aldosterone'' and ''18-hydroxycorticosterone'' values for patients with essential hypertension and normal aldosterone excretion were also (moderately) increased. Thus, when high values are found, true aldosterone values must be estimated by extraction and chromatography, to eliminate false positives. The ''aldosterone'' and ''18-hydroxycorticosterone'' values by our procedure are much higher than the corresponding values for urinary free aldosterone and 18-hydroxycorticosterone. Although not identified, the immunoactive materials are probably metabolites of aldosterone and 18-hydroxycorticosterone.

• OSTI ID: 5053080
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:3; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARDIOVASCULAR DISEASES
CORTICOSTEROIDS
DIAGNOSIS
DISEASES
ENDOCRINE DISEASES
HORMONES
HYDROXY COMPOUNDS
HYPERTENSION
ISOTOPE APPLICATIONS
KETONES
MATERIALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
PATIENTS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
STEROID HORMONES
STEROIDS
SYMPTOMS
TRACER TECHNIQUES
URINE
VASCULAR DISEASES
WASTES