Radioimmunoassay for aldosterone without chromatography. Determination of urinary 18-glucuronide excretion
Journal Article
·
· Acta Endocrinol., v. 74, pp. 548-557
OSTI ID:4340514
A radioimmunoassay for the determination of urinary aldosterone-18- glucuronide was developed for the clinical laboratory. Ten ml of urine is pre- extracted with methylene chloride and then submitted to acid hydrolysis. This is followed by a second methylene chloride extraction. The aldosterone concentration in these extracts is determined with antisera obtained from rabbits immunized with an aldosterone-oxime bovine gamma globulin conjugate, In view of the high recovery of STA1,2/sup -3/H! aldosterone, no internal indicator standands were used. The sensitivity of the overall assay was 0.44 mu g/24 h. The coefficient of variation was 3.7% within a single assay and 13.8% for multiple assays. The specificity of the method was examined by comparison with a double isotope dilution derivative method and by simultaneous use of six different antisera. Under normal sodium intake the urinary excretion of the acid- labile aldosterone-18-glucuronide ranged from 4.0 to 13.0 mu g 24 h in nine normal male subjects. (auth)
- Research Organization:
- Univ., Zurich
- NSA Number:
- NSA-29-016105
- OSTI ID:
- 4340514
- Journal Information:
- Acta Endocrinol., v. 74, pp. 548-557, Journal Name: Acta Endocrinol., v. 74, pp. 548-557; ISSN ACENA
- Country of Publication:
- Denmark
- Language:
- English
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