Direct radioimmunoassay for aldosterone in unextracted serum and plasma

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5799375

Lee, T P; Tan, C H

A novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme is described. Heating at 75/sup o/C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to inactivate the enzyme. The antibody-bound fraction is then precipitated with polyethylene glycol and isolated by centrifugation. This easy method eliminates extraction and purification and gives accurate and reliable results. The total time required for 100 estimations, including counting time, is about 6 h.

• Research Organization: National Univ. of Singapore
• OSTI ID: 5799375
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:12; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ALDEHYDES
ALDOSTERONE
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BLOOD SERUM
BODY FLUIDS
CELLULOLYTIC ACTIVITY
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
DATA
DIGESTION
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
HORMONES
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KETONES
MATERIALS
MEASURING METHODS
MINERALOCORTICOIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RESPONSE MODIFYING FACTORS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES