Direct solid-phase /sup 125/I radioimmunoassay of serum cortisol
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:6453424
We report a solid-phase iodine-125 radioimmunoassay for serum cortisol in which the interfering binding proteins are inactivated by a combination of reaction pH and 8-anilino-1-naphthalenesulfonic acid. The procedure is easy to perform and gives accurate and reliable results. The shift in pH for optimum binding of an antibody resulting from immobilization on a solid support, used to decrease cortisol binding competition in the present test, is potentially exploitable in other antibody and enzyme systems.
- Research Organization:
- Corning Medical Diagnostics, Medfield, MA
- OSTI ID:
- 6453424
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:9; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
/sup 125/I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured
Direct radioimmunoassay for aldosterone in unextracted serum and plasma
Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum
Journal Article
·
Sun Oct 31 23:00:00 EST 1976
· Clin. Chem.; (United States)
·
OSTI ID:7226299
Direct radioimmunoassay for aldosterone in unextracted serum and plasma
Journal Article
·
Mon Nov 30 23:00:00 EST 1981
· Clin. Chem. (Winston-Salem, N.C.); (United States)
·
OSTI ID:5799375
Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum
Journal Article
·
Tue Jun 01 00:00:00 EDT 1982
· Clin. Chem. (Winston-Salem, N.C.); (United States)
·
OSTI ID:7056026
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACCURACY
ADRENAL HORMONES
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACCURACY
ADRENAL HORMONES
BETA DECAY RADIOISOTOPES
BLOOD SERUM
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES