/sup 125/I-labeled cortisol radioimmunoassay in which serum binding protein are enzymatically denatured

Journal Article · Mon Nov 01 04:00:00 EST 1976 · Clin. Chem.; (United States)

OSTI ID:7226299

Hasler, M J; Painter, K; Niswender, G D

We report an iodine-125 radioimmunoassay for cortisol in biological fluids, in which interfering binding proteins are enzymatically denatured. An antiserum to cortisol-3-carboxymethyloxime-bovine serum albumin, extremely low cross-reacting with other corticosteroids, was raised in rabbits. A cortisol-3-carboxymethyloxime tyrosine methyl ester derivative was synthesized and labeled with iodine-125 by standard radioiodination techniques. To eliminate the need for extraction and recovery procedures, we digested interfering binding with a proteolytic enzyme, which then was heat-inactivated before adding the labeled derivative and the premixed, preincubated antiserum complex. There was quantitative analytical recovery of esogenous cortisol added to sera from a normal man, a normal woman, and a pregnant woman. Values for the same samples agreed after extraction and chromatographic purification and agreed well with values obtained by other techniques by independent reference laboratories. The five-step assay can be done in 6 h or less.

• Research Organization: Mircomedic Diagnostics, Inc., Fort Collins, CO
• OSTI ID: 7226299
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 22:11; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ANIMALS
BETA DECAY RADIOISOTOPES
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LABELLED COMPOUNDS
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
PRIMATES
PROTEIN DENATURATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES