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Simple radioimmunoassay of cortisol in diluted samples of human plasma. [Tritium tracer technique]

Journal Article · · Clin. Chem.; (United States)
OSTI ID:6340476
;

We describe a simple radioimmunoassay of plasma cortisol, which can be performed in 3 h and which requires no purification, heating, or refrigeration steps. The plasma proteins are inhibited through direct competition between them and the antiserum at room temperature, at which the antiserum's affinity exceed that of the binding proteins. Plasma, diluted with water, is incubated for 3 h at room temperature with (/sup 3/H) cortisol and the antiserum. We compared results with those by the usual extraction method. The correlation between methods on evaluating normal samples with one antiserum was ..gamma.. = 0.954 (P < 0.001), and the slope was 0.661. With three other antisera it was ..gamma.. = 0.922 (P < 0.001) and slope 0.644. Plasmas with abnormal protein concentrations (i.e., from pregnant women, and after corticotropin adminsitration), tested to examine the validity of the method for routine use, and to define the role of the protein carriers, showed ..gamma.. = 0.859 (P < 0.001) and slope 0.726 for the four antisera used. Additional samples, assayed with diluted standards plus stripped plasma, showed a correlation with the usual extraction method of ..gamma.. = 0.945 (P < 0.001) and slope 1.026.

Research Organization:
Univ. of Heidelberg, West Ger.
OSTI ID:
6340476
Journal Information:
Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:9; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CORTICOSTEROIDS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES