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Improved radioimmunoassay of urinary estriol. [/sup 125/I tracer technique]

Journal Article · · Clin. Chem.; (United States)
OSTI ID:6530972
We reported a rapid double-antibody radioimmunoassay for urinary estriol. Advantages over other current methods include: 30-min hydrolysis; total incubation time, 55 min; assay unaffected by urinary glucose; no degradation of estriol evident during hydrolysis; superior (85%) analytical recovery of estriol conjugates; linear standard curve by logit-log extrapolation; good correlation (r = 0.83) with total estrogen determination by a generally accepted colorimetric method; only 20 ..mu..L of urine required; and the detection range is 1.9 to 100.5 mg/24-h urine.
Research Organization:
Univ. of Kentucky Coll. of Medicine, Lexington
OSTI ID:
6530972
Journal Information:
Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 25:1; ISSN CLCHA
Country of Publication:
United States
Language:
English